Lunar glass beads, formed through impact and volcanic processes, are used in SIMS U-Pb dating to provide valuable insights into the Moon’s volcanic activity and impact history. However, lunar glass beads exhibit a wide range of chemical compositions deviating from the commonly used glass reference materials. The lack of glass reference materials with high FeO content presents a challenge for correcting matrix effects in SIMS analyses of impact glasses with variable FeO contents. This study prepared and preliminarily characterized a new synthetic glass reference material (LMG-4) with compositions analogous to those of Chang’e-5 soils and mare basalts, plotting in the center of the published compositional range of Chang’e-5 impact glass beads. This glass exhibits significantly higher FeO and lower SiO2 contents than other available reference glasses, fulfilling the requirements for high-FeO glass reference materials. U-Pb-Si-O isotope ratios and Fe2+/ΣFe ratio were determined using bulk ID-TIMS, MC-ICP-MS, IRMS, and potassium dichromate volumetric method. The homogeneity of LMG-4 is confirmed through EPMA and SIMS spot analyses. This new synthetic glass reference material offers a potential solution for correcting matrix effects in U-Pb-Si-O isotope analyses of high-FeO glass using in situ microanalytical techniques.