Zinc (Zn) isotopes are increasingly applied in biogeochemistry, nutrition, and environmental sciences, but systematic data for biological reference materials remain scarce. In this study, we present high-precision Zn isotope measurements of a wide range of certified biological reference materials, including plants, animal tissues, human tissues, and processed food, using multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS) combined with a one-column ion-exchange purification protocol. The analytical procedure yielded excellent repeatability, with long-term precision better than ± 0.07 ‰ (2SD) for δ66/64Zn. Isotopic analyses were performed on 23 certified biological reference materials, covering plants (e.g., wheat, spinach leaves, ginseng), animal/human tissues (e.g., scallop, pork liver, human hair), and processed foods (e.g., milk powder, infant formula). The measured δ66/64Zn values span distinct ranges: plants (0.19‰ to 0.84 ‰), animal tissues (-0.61 ‰ to 0.35 ‰), human hair (-0.07 ‰ to -0.08 ‰), and food products (0.49 ‰ to 0.88 ‰). These results were consistent with previously reported data for selected standards (e.g., BCR-2, BHVO-2, GBW10051), confirming method accuracy. Importantly, this study provides the first systematic dataset of δ66/64Zn values for 18 previously unreported biological reference materials, thereby establishing robust baselines for future research in Zn biogeochemical cycling, food authenticity, nutritional assessment, paleodiet and biomedical applications.