Molybdenum (Mo) isotopic analysis using multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS) has become a cornerstone technique in modern geochemistry. However, achieving accurate and precise measurements in samples with low Mo concentrations or complex matrices remains challenging due to conventional instrument limitations. This study presents the first systematic evaluation of the Thermo Scientific Neoma MS/MS MC-ICP-MS, a new and advanced platform featuring a double-Wien filter and an advanced collision/reaction cell (CRC) design, for Mo isotope measurements. We investigated how instrumental configurations—specifically cone combinations and plasma conditions—affect sensitivity and matrix tolerance, focusing on its potential to overcome existing analytical barriers. Compare to Neptune platform, the Neoma MS/MS exhibits markedly enhanced sensitivity, yielding a 2- to 5-fold improvement, under both dry and wet plasma conditions. Critically, a long-term external precision of 0.06‰ (2SD) at low concentrations, down to 10 ng g-1, was achieved via Neoma. Notably, the Jet/X cone interface simultaneously enhances signal intensity and mitigates matrix effect, successfully challenging the conventional trade-off between sensitivity and matrix effect. These results highlight the Neoma MS/MS as a significant advancement for high-precision Mo isotope analysis, offering unprecedented accuracy and resilience under demanding geological conditions, particularly for samples with extremely low Mo content.