Abstract:A digestion-free graphite furnace atomic absorption spectrometry (GFAAS) method was developed for the simultaneous determination of total As and Se in fruit juices using a transversely heated graphite atomizer (THGA) with end-capped pyrolytic graphite tubes fitted with integrated graphite platforms (IGPs). For the analysis, 5 μL of oxidizing reagent (10% (v/v) HNO3 and 60% (v/v) H2O2) with 4 μL of 5 g/L Pd and 5 μL of 0.5 g/L Mg chemical modifier (both applied as nitrates) was directly injected onto the preheated IGP of the THGA (70 oC) along with 5 μL of juice sample. Optimum pyrolysis and atomization temperatures were 1300°C and 2100°C, respectively. Sensitivity of the method, expressed as the characteristic mass, was 22 pg As and 36 pg Se, in quite good agreement with model calculations (19 pg As and 27 pg Se). LODs were 2.1 μg/L (As) and 2.5 μg/L (Se), well below the European Food Safety Authority (EFSA) limits (20 μg/L). The calibration curves of both As and Se were linear up to 150 μg/L. Method validation with sixteen spiked juice samples yielded average recoveries of 93% for both As and Se. The As and Se contents of all juice samples (apple, grape, orange, peach, and mixed fruit) were below the LOD. A conservative dietary exposure assessment using upper-bound assumptions indicated negligible health risk for both elements. The method provides a rapid, green, and reliable approach for routine screening of As and Se in fruit juices.