Clinopyroxene is a major reservoir for Sr and rare earth elements (REE) in the lithospheric mantle and exerts long-term control on mantle Sr isotopic compositions. High-precision in situ Sr isotopic analysis of clinopyroxene by LA-MC-ICPMS requires matrix-matched reference materials with high Sr contents. However, suitable clinopyroxene reference materials remain scarce, limiting the wider application of this technique. Furthermore, the potential effects of non-matrix-matched calibration on data accuracy have been rarely evaluated. In this study, we present a comprehensive characterization of a potential new high-Sr clinopyroxene reference material, ZYC01, using multiple analytical techniques. ZYC01 exhibits excellent homogeneity in Sr isotopes. It has the highest Sr content (~2000 μg g?1) among all currently reported clinopyroxene reference materials, along with extremely low Rb concentrations. TIMS analyses yield a mean 87Sr/86Sr ratio of 0.705946 ± 0.000006 (2s, MSWD = 0.4; n = 8), while LA-MC-ICPMS analyses give a mean of 0.705956 ± 0.000080 (2s, MSWD = 1.1; n = 308), confirming its suitability as a Sr isotope reference material. Systematic comparison of matrix-matched versus non-matrix-matched calibration for ZYC01 clinopyroxene reveals that synthetic glasses (BCR-2G, BHVO-2G) produce systematic offsets exceeding 0.0004 relative to the TIMS value, while apatite calibration yields a ~0.00006 bias. In contrast, matrix-matched calibration using clinopyroxene YY09-47 gives results in excellent agreement with TIMS. These findings confirm that accurate in situ Sr isotopic analysis of clinopyroxene necessitates rigorous matrix matching. ZYC01 represents a valuable addition to the limited suite of high-Sr clinopyroxene reference materials and will facilitate wider application of in situ Sr isotopic analysis in mantle geochemistry.